Mapping of Hd-6-2 for Heading Date Using Two Secondary Segregation Populations in Rice

doi:10.1016/j.rsci.2018.02.005

Abstract

Abstract:

Heading date is one of the most important traits for rice adaption to different cultivation areas and crop seasons. In this study, two single segment substitution lines (SSSLs), W31-41-61-3-11-3-6-7 (W31-SSSL) and W32-59-80-2-11-1-10 (W32-SSSL) with substituted intervals derived from the donor parents IR66897B (W31) and IR66167-27-5-1-6 (W32), respectively, with Huajingxian 74 (HTX74) were found to comprise a gene for extremely late-heading date, and the gene was tentatively designated as Hd-6-2. Two secondary F₂ segregating populations were developed by crossing the two heterozygous SSSLs with HJX74 to map Hd-6-2 gene. According to phenotype analysis of the two mapping populations, the late heading date trait was controlled by a major recessive gene. In the segregation population derived from W31-SSSL, Hd-6-2 was mapped on chromosome 6 between PSM677 and RM204 with the genetic distances of 1.3 and 2.7 cM, respectively. In the population of W32-SSSL, the gene for heading date was mapped to the similar region as Hd-6-2 and co-segregated with PSM672. The sequence alignment of Hd3a in the coding domains and promoter regions of HJX74 and W31-SSSL are completely consistent, whereas there was a great difference between W32-SSSL and HJX74, suggesting that Hd3a could hardly be the main cause of the heading date variation in W31-SSSL, but it was probably the main reason for the change of heading stage in W32-SSSL.

Key words: rice, single segment substitution line, heading date, gene mapping, Hd3a

Hua Zhang, Xu Liu, Yongyi Yang, Ning Xuan, Fangyin Yao. Mapping of Hd-6-2 for Heading Date Using Two Secondary Segregation Populations in Rice[J]. Rice Science, 2018, 25(3): 161-168.

Figures/Tables 5

Fig. 1. Distribution of heading date based on F2 segregating populations of W31-SSSL (A) and W32-SSSL (B).W31-SSSL, W32-SSSL and HJX74 headed at (132.3 ± 1.4) d, (135.5 ± 1.4) d and (104.5 ± 1.7) d, respectively.Bar represents standard deviation.

Table 1 Co-segregating of genotypes for heading date in two populations.

Population	Marker	Genotype 1	Genotype 2	Genotype 3	χ²_0.05 (3:1)
W31-SSSL (197 lines)	RM587	38	107	52	3.47
	RM510	40	102	55	1.53
	RM204	42	100	55	1.76
	RM225	42	98	57	2.29
	PSM677	40	102	55	1.53
	PSM678	40	102	55	1.53
W32-SSSL (201 lines)	RM587	54	89	58	2.79
	PSM672	53	90	58	2.44
	RM204	53	90	58	2.44
	PSM676	52	90	59	2.68
	PSM678	54	89	58	2.79
Genotype 1 is identical to the receipt; Genotype 2 is heterozygous; and Genotype 3 is identical to the donor.

Fig. 2. Linkage map of Hd-6-2 locus for heading date using the segregating populations from W31-SSSL (A) and W32-SSSL (B).Numbers on the left are genetic distances between the upper and below markers.

Fig. 3. Relative expression level of Hd3a in HJX74, W31-SSSL and W32-SSSL.Bars represent standard deviation.

Fig. 4. Alignments of Hd3a coding domains (CDS) sequences and amino acid (AA) sequences in HJX74 and W32-SSSL.

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